Construct: shRNA TRCN0000029210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005155.5-1270s1c1
DNA Barcode:
GCAGTCCAGCTTCTTTGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPT2 (9374)
Gene Description:
palmitoyl-protein thioesterase 2
Transcript:
RefSeq NM_005155.5 (NON-CURRENT)
Match location:
Position 1270 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCCAGCTTCTTTGGTTT
Hairpin Sequence:
5'-CCGG-GCAGTCCAGCTTCTTTGGTTT-CTCGAG-AAACCAAAGAAGCTGGACTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCCAGCTTCTTTGGTTTCTCGAGAAACCAAAGAAGCTGGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCCAGCTTCTTTGGTTTCTCGAGAAACCAAAGAAGCTGGACTGC-3'

Other clones with same target sequence:

(none)