Construct: shRNA TRCN0000029211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005155.5-923s1c1
DNA Barcode:
GCTCTGCTTTCTGTCATGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPT2 (9374)
Gene Description:
palmitoyl-protein thioesterase 2
Transcript:
RefSeq NM_005155.5 (NON-CURRENT)
Match location:
Position 923 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGCTTTCTGTCATGGAT
Hairpin Sequence:
5'-CCGG-GCTCTGCTTTCTGTCATGGAT-CTCGAG-ATCCATGACAGAAAGCAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGCTTTCTGTCATGGATCTCGAGATCCATGACAGAAAGCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGCTTTCTGTCATGGATCTCGAGATCCATGACAGAAAGCAGAGC-3'

Other clones with same target sequence:

(none)