Construct: shRNA TRCN0000029235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000203.2-598s1c1
DNA Barcode:
GCATGTTTCCAAGTGGAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IDUA (3425)
Gene Description:
alpha-L-iduronidase
Transcript:
RefSeq NM_000203.2 (NON-CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTTTCCAAGTGGAACTT
Hairpin Sequence:
5'-CCGG-GCATGTTTCCAAGTGGAACTT-CTCGAG-AAGTTCCACTTGGAAACATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTTTCCAAGTGGAACTTCTCGAGAAGTTCCACTTGGAAACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTTTCCAAGTGGAACTTCTCGAGAAGTTCCACTTGGAAACATGC-3'

Other clones with same target sequence:

(none)