Construct: shRNA TRCN0000029252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005506.2-1219s1c1
DNA Barcode:
CGGTATAAAGTTCCTGCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCARB2 (950)
Gene Description:
scavenger receptor class B member 2
Transcript:
RefSeq NM_005506.2 (NON-CURRENT)
Match location:
Position 1219 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTATAAAGTTCCTGCAGAA
Hairpin Sequence:
5'-CCGG-CGGTATAAAGTTCCTGCAGAA-CTCGAG-TTCTGCAGGAACTTTATACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTATAAAGTTCCTGCAGAACTCGAGTTCTGCAGGAACTTTATACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTATAAAGTTCCTGCAGAACTCGAGTTCTGCAGGAACTTTATACCG-3'

Other clones with same target sequence:

  1. TRCN0000352967