Construct: shRNA TRCN0000029254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005606.3-735s1c1
DNA Barcode:
GCCGGATAACATCAATGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LGMN (5641)
Gene Description:
legumain
Transcript:
RefSeq NM_005606.3 (NON-CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGATAACATCAATGTTTA
Hairpin Sequence:
5'-CCGG-GCCGGATAACATCAATGTTTA-CTCGAG-TAAACATTGATGTTATCCGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGATAACATCAATGTTTACTCGAGTAAACATTGATGTTATCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGATAACATCAATGTTTACTCGAGTAAACATTGATGTTATCCGGC-3'

Other clones with same target sequence:

(none)