Construct: shRNA TRCN0000029322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006432.3-377s1c1
DNA Barcode:
TGATGGTTGTAAGAGTGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPC2 (10577)
Gene Description:
NPC intracellular cholesterol transporter 2
Transcript:
RefSeq NM_006432.3 (NON-CURRENT)
Match location:
Position 377 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGGTTGTAAGAGTGGAAT
Hairpin Sequence:
5'-CCGG-TGATGGTTGTAAGAGTGGAAT-CTCGAG-ATTCCACTCTTACAACCATCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGGTTGTAAGAGTGGAATCTCGAGATTCCACTCTTACAACCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGGTTGTAAGAGTGGAATCTCGAGATTCCACTCTTACAACCATCA-3'

Other clones with same target sequence:

  1. TRCN0000293235