Construct: shRNA TRCN0000029336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003566.1-1313s1c1
DNA Barcode:
GCGGAGTTTAAGCAGCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEA1 (8411)
Gene Description:
early endosome antigen 1
Transcript:
RefSeq NM_003566.1 (NON-CURRENT)
Match location:
Position 1313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGTTTAAGCAGCTACAA
Hairpin Sequence:
5'-CCGG-GCGGAGTTTAAGCAGCTACAA-CTCGAG-TTGTAGCTGCTTAAACTCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGTTTAAGCAGCTACAACTCGAGTTGTAGCTGCTTAAACTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGTTTAAGCAGCTACAACTCGAGTTGTAGCTGCTTAAACTCCGC-3'

Other clones with same target sequence:

(none)