Construct: shRNA TRCN0000029362

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031205.2-448s1c1
DNA Barcode:
GTCCCAGCAGATCAACATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CABP1 (9478)
Gene Description:
calcium binding protein 1
Transcript:
RefSeq NM_031205.2 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029362)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCCAGCAGATCAACATGAA
Hairpin Sequence:
5'-CCGG-GTCCCAGCAGATCAACATGAA-CTCGAG-TTCATGTTGATCTGCTGGGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCCAGCAGATCAACATGAACTCGAGTTCATGTTGATCTGCTGGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCCAGCAGATCAACATGAACTCGAGTTCATGTTGATCTGCTGGGAC-3'

Other clones with same target sequence:

(none)