Construct: shRNA TRCN0000029367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001688.2-235s1c1
DNA Barcode:
TCTTCCTGAATACGGAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP5PB (515)
Gene Description:
ATP synthase peripheral stalk-membrane subunit b
Transcript:
RefSeq NM_001688.2 (NON-CURRENT)
Match location:
Position 235 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCCTGAATACGGAGGAAA
Hairpin Sequence:
5'-CCGG-TCTTCCTGAATACGGAGGAAA-CTCGAG-TTTCCTCCGTATTCAGGAAGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCCTGAATACGGAGGAAACTCGAGTTTCCTCCGTATTCAGGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCCTGAATACGGAGGAAACTCGAGTTTCCTCCGTATTCAGGAAGA-3'

Other clones with same target sequence:

(none)