Construct: shRNA TRCN0000029405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003406.2-738s1c1
DNA Barcode:
GCAATTACTGAGAGACAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YWHAZ (7534)
Gene Description:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
Transcript:
RefSeq NM_003406.2 (NON-CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATTACTGAGAGACAACTT
Hairpin Sequence:
5'-CCGG-GCAATTACTGAGAGACAACTT-CTCGAG-AAGTTGTCTCTCAGTAATTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATTACTGAGAGACAACTTCTCGAGAAGTTGTCTCTCAGTAATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATTACTGAGAGACAACTTCTCGAGAAGTTGTCTCTCAGTAATTGC-3'

Other clones with same target sequence:

  1. TRCN0000292168