Construct: shRNA TRCN0000029413

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101.2-898s1c1
DNA Barcode:
CGAAACTACCTTCAACTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACTB (60)
Gene Description:
actin beta
Transcript:
RefSeq NM_001101.2 (NON-CURRENT)
Match location:
Position 898 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029413)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAACTACCTTCAACTCCAT
Hairpin Sequence:
5'-CCGG-CGAAACTACCTTCAACTCCAT-CTCGAG-ATGGAGTTGAAGGTAGTTTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAACTACCTTCAACTCCATCTCGAGATGGAGTTGAAGGTAGTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAACTACCTTCAACTCCATCTCGAGATGGAGTTGAAGGTAGTTTCG-3'

Other clones with same target sequence:

  1. TRCN0000349687