Construct: shRNA TRCN0000029419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_208213.3-616s1c1
DNA Barcode:
CCTGACTATATGAAGGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP4A22 (284541)
Gene Description:
cytochrome P450 family 4 subfamily A member 22
Transcript:
RefSeq XM_208213.3 (NON-CURRENT)
Match location:
Position 616 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACTATATGAAGGTGATT
Hairpin Sequence:
5'-CCGG-CCTGACTATATGAAGGTGATT-CTCGAG-AATCACCTTCATATAGTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACTATATGAAGGTGATTCTCGAGAATCACCTTCATATAGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACTATATGAAGGTGATTCTCGAGAATCACCTTCATATAGTCAGG-3'

Other clones with same target sequence:

(none)