Construct: shRNA TRCN0000029500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006431.1-1527s1c1
DNA Barcode:
CCTGGGTATAACAGAAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCT2 (10576)
Gene Description:
chaperonin containing TCP1 subunit 2
Transcript:
RefSeq NM_006431.1 (NON-CURRENT)
Match location:
Position 1527 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGTATAACAGAAAGTTT
Hairpin Sequence:
5'-CCGG-CCTGGGTATAACAGAAAGTTT-CTCGAG-AAACTTTCTGTTATACCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGTATAACAGAAAGTTTCTCGAGAAACTTTCTGTTATACCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGTATAACAGAAAGTTTCTCGAGAAACTTTCTGTTATACCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000275594