Construct: shRNA TRCN0000029532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000463.2-774s1c1
DNA Barcode:
CTGGCTGTTTAGAAGTGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT1A1 (54658)
Gene Description:
UDP glucuronosyltransferase family 1 member A1
Transcript:
RefSeq NM_000463.2 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGCTGTTTAGAAGTGACTT
Hairpin Sequence:
5'-CCGG-CTGGCTGTTTAGAAGTGACTT-CTCGAG-AAGTCACTTCTAAACAGCCAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGCTGTTTAGAAGTGACTTCTCGAGAAGTCACTTCTAAACAGCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGCTGTTTAGAAGTGACTTCTCGAGAAGTCACTTCTAAACAGCCAG-3'

Other clones with same target sequence:

(none)