Construct: shRNA TRCN0000029600

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002001.2-204s1c1
DNA Barcode:
CCCTCCATGGAATAGAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FCER1A (2205)
Gene Description:
Fc fragment of IgE receptor Ia
Transcript:
RefSeq NM_002001.2 (NON-CURRENT)
Match location:
Position 204 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029600)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCATGGAATAGAATATT
Hairpin Sequence:
5'-CCGG-CCCTCCATGGAATAGAATATT-CTCGAG-AATATTCTATTCCATGGAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCATGGAATAGAATATTCTCGAGAATATTCTATTCCATGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCATGGAATAGAATATTCTCGAGAATATTCTATTCCATGGAGGG-3'

Other clones with same target sequence:

(none)