Construct: shRNA TRCN0000029628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000573.2-6081s1c1
DNA Barcode:
CCTCTCTTGGATAATTCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CR1 (1378)
Gene Description:
complement C3b/C4b receptor 1 (Knops blood group)
Transcript:
RefSeq NM_000573.2 (NON-CURRENT)
Match location:
Position 6081 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCTTGGATAATTCTAAA
Hairpin Sequence:
5'-CCGG-CCTCTCTTGGATAATTCTAAA-CTCGAG-TTTAGAATTATCCAAGAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCTTGGATAATTCTAAACTCGAGTTTAGAATTATCCAAGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCTTGGATAATTCTAAACTCGAGTTTAGAATTATCCAAGAGAGG-3'

Other clones with same target sequence:

(none)