Construct: shRNA TRCN0000029648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002211.2-350s1c1
DNA Barcode:
GCCCTCCAGATGACATAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITGB1 (3688)
Gene Description:
integrin subunit beta 1
Transcript:
RefSeq NM_002211.2 (NON-CURRENT)
Match location:
Position 350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCCAGATGACATAGAAA
Hairpin Sequence:
5'-CCGG-GCCCTCCAGATGACATAGAAA-CTCGAG-TTTCTATGTCATCTGGAGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCCAGATGACATAGAAACTCGAGTTTCTATGTCATCTGGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCCAGATGACATAGAAACTCGAGTTTCTATGTCATCTGGAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000275135