Construct: shRNA TRCN0000029662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006770.2-202s1c1
DNA Barcode:
CCAAATTGCAATGGAGCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCO (8685)
Gene Description:
macrophage receptor with collagenous structure
Transcript:
RefSeq NM_006770.2 (NON-CURRENT)
Match location:
Position 202 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATTGCAATGGAGCCTTT
Hairpin Sequence:
5'-CCGG-CCAAATTGCAATGGAGCCTTT-CTCGAG-AAAGGCTCCATTGCAATTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATTGCAATGGAGCCTTTCTCGAGAAAGGCTCCATTGCAATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATTGCAATGGAGCCTTTCTCGAGAAAGGCTCCATTGCAATTTGG-3'

Other clones with same target sequence:

(none)