Construct: shRNA TRCN0000029671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002438.1-4243s1c1
DNA Barcode:
CCCTTCTAAACCGTCTTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRC1 (4360)
Gene Description:
mannose receptor C-type 1
Transcript:
RefSeq NM_002438.1 (NON-CURRENT)
Match location:
Position 4243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCTAAACCGTCTTCCAA
Hairpin Sequence:
5'-CCGG-CCCTTCTAAACCGTCTTCCAA-CTCGAG-TTGGAAGACGGTTTAGAAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCTAAACCGTCTTCCAACTCGAGTTGGAAGACGGTTTAGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCTAAACCGTCTTCCAACTCGAGTTGGAAGACGGTTTAGAAGGG-3'

Other clones with same target sequence:

(none)