Construct: shRNA TRCN0000029677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006039.2-3125s1c1
DNA Barcode:
CCCAATGTGACCTTTGACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRC2 (9902)
Gene Description:
mannose receptor C type 2
Transcript:
RefSeq NM_006039.2 (NON-CURRENT)
Match location:
Position 3125 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGTGACCTTTGACCTT
Hairpin Sequence:
5'-CCGG-CCCAATGTGACCTTTGACCTT-CTCGAG-AAGGTCAAAGGTCACATTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGTGACCTTTGACCTTCTCGAGAAGGTCAAAGGTCACATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGTGACCTTTGACCTTCTCGAGAAGGTCAAAGGTCACATTGGG-3'

Other clones with same target sequence:

(none)