Construct: shRNA TRCN0000029690

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021155.2-486s1c1
DNA Barcode:
GCTTCCAGAGAAATCTAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD209 (30835)
Gene Description:
CD209 molecule
Transcript:
RefSeq NM_021155.2 (NON-CURRENT)
Match location:
Position 486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029690)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCAGAGAAATCTAAGAT
Hairpin Sequence:
5'-CCGG-GCTTCCAGAGAAATCTAAGAT-CTCGAG-ATCTTAGATTTCTCTGGAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCAGAGAAATCTAAGATCTCGAGATCTTAGATTTCTCTGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCAGAGAAATCTAAGATCTCGAGATCTTAGATTTCTCTGGAAGC-3'

Other clones with same target sequence:

(none)