Construct: shRNA TRCN0000029730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001623.3-281s1c1
DNA Barcode:
CCTGAAACGAATGCTGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AIF1 (199)
Gene Description:
allograft inflammatory factor 1
Transcript:
RefSeq NM_001623.3 (NON-CURRENT)
Match location:
Position 281 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAACGAATGCTGGAGAA
Hairpin Sequence:
5'-CCGG-CCTGAAACGAATGCTGGAGAA-CTCGAG-TTCTCCAGCATTCGTTTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAACGAATGCTGGAGAACTCGAGTTCTCCAGCATTCGTTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAACGAATGCTGGAGAACTCGAGTTCTCCAGCATTCGTTTCAGG-3'

Other clones with same target sequence:

(none)