Construct: shRNA TRCN0000029747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002861.1-689s1c1
DNA Barcode:
GCAGAGACCAACCAGAATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF5 (7984)
Gene Description:
Rho guanine nucleotide exchange factor 5
Transcript:
RefSeq NM_001002861.1 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGACCAACCAGAATGAA
Hairpin Sequence:
5'-CCGG-GCAGAGACCAACCAGAATGAA-CTCGAG-TTCATTCTGGTTGGTCTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGACCAACCAGAATGAACTCGAGTTCATTCTGGTTGGTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGACCAACCAGAATGAACTCGAGTTCATTCTGGTTGGTCTCTGC-3'

Other clones with same target sequence:

(none)