Construct: shRNA TRCN0000029793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021159.3-1406s1c1
DNA Barcode:
GCTGAACAATTGGGAAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAP1GDS1 (5910)
Gene Description:
Rap1 GTPase-GDP dissociation stimulator 1
Transcript:
RefSeq NM_021159.3 (NON-CURRENT)
Match location:
Position 1406 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAACAATTGGGAAAGAAT
Hairpin Sequence:
5'-CCGG-GCTGAACAATTGGGAAAGAAT-CTCGAG-ATTCTTTCCCAATTGTTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAACAATTGGGAAAGAATCTCGAGATTCTTTCCCAATTGTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAACAATTGGGAAAGAATCTCGAGATTCTTTCCCAATTGTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000344337