Construct: shRNA TRCN0000029800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006065.1-70s1c1
DNA Barcode:
TCCTAGTCCTTTCCTGCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIRPB1 (10326)
Gene Description:
signal regulatory protein beta 1
Transcript:
RefSeq NM_006065.1 (NON-CURRENT)
Match location:
Position 70 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTAGTCCTTTCCTGCTGAT
Hairpin Sequence:
5'-CCGG-TCCTAGTCCTTTCCTGCTGAT-CTCGAG-ATCAGCAGGAAAGGACTAGGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTAGTCCTTTCCTGCTGATCTCGAGATCAGCAGGAAAGGACTAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTAGTCCTTTCCTGCTGATCTCGAGATCAGCAGGAAAGGACTAGGA-3'

Other clones with same target sequence:

(none)