Construct: shRNA TRCN0000029808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018556.2-457s1c1
DNA Barcode:
GAGATGGCTTTGGGTGCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIRPG (55423)
Gene Description:
signal regulatory protein gamma
Transcript:
RefSeq NM_018556.2 (NON-CURRENT)
Match location:
Position 457 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATGGCTTTGGGTGCCAAA
Hairpin Sequence:
5'-CCGG-GAGATGGCTTTGGGTGCCAAA-CTCGAG-TTTGGCACCCAAAGCCATCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATGGCTTTGGGTGCCAAACTCGAGTTTGGCACCCAAAGCCATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATGGCTTTGGGTGCCAAACTCGAGTTTGGCACCCAAAGCCATCTC-3'

Other clones with same target sequence:

(none)