Construct: shRNA TRCN0000029844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010164.1-2020s1c1
DNA Barcode:
GATGTACCAATTTCAGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eya1 (14048)
Gene Description:
EYA transcriptional coactivator and phosphatase 1
Transcript:
RefSeq NM_010164.1 (NON-CURRENT)
Match location:
Position 2020 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTACCAATTTCAGCATAT
Hairpin Sequence:
5'-CCGG-GATGTACCAATTTCAGCATAT-CTCGAG-ATATGCTGAAATTGGTACATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTACCAATTTCAGCATATCTCGAGATATGCTGAAATTGGTACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTACCAATTTCAGCATATCTCGAGATATGCTGAAATTGGTACATC-3'

Other clones with same target sequence:

(none)