Construct: shRNA TRCN0000029867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008905.1-2206s1c1
DNA Barcode:
CTCAGCATCAAATGTGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppfibp2 (19024)
Gene Description:
PTPRF interacting protein, binding protein 2 (liprin beta 2)
Transcript:
RefSeq NM_008905.1 (NON-CURRENT)
Match location:
Position 2206 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGCATCAAATGTGCTATT
Hairpin Sequence:
5'-CCGG-CTCAGCATCAAATGTGCTATT-CTCGAG-AATAGCACATTTGATGCTGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGCATCAAATGTGCTATTCTCGAGAATAGCACATTTGATGCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGCATCAAATGTGCTATTCTCGAGAATAGCACATTTGATGCTGAG-3'

Other clones with same target sequence:

  1. TRCN0000353485