Construct: shRNA TRCN0000029871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008975.2-899s1c1
DNA Barcode:
GCGCTTCCTCATCACCCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptp4a3 (19245)
Gene Description:
protein tyrosine phosphatase 4a3
Transcript:
RefSeq NM_008975.2 (NON-CURRENT)
Match location:
Position 899 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTTCCTCATCACCCACAA
Hairpin Sequence:
5'-CCGG-GCGCTTCCTCATCACCCACAA-CTCGAG-TTGTGGGTGATGAGGAAGCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTTCCTCATCACCCACAACTCGAGTTGTGGGTGATGAGGAAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTTCCTCATCACCCACAACTCGAGTTGTGGGTGATGAGGAAGCGC-3'

Other clones with same target sequence:

  1. TRCN0000319972