Construct: shRNA TRCN0000029880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011203.2-269s1c1
DNA Barcode:
CCGAGTTAAGTTGACTTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpn12 (19248)
Gene Description:
protein tyrosine phosphatase, non-receptor type 12
Transcript:
RefSeq NM_011203.2 (CURRENT)
Match location:
Position 269 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTTAAGTTGACTTTGAA
Hairpin Sequence:
5'-CCGG-CCGAGTTAAGTTGACTTTGAA-CTCGAG-TTCAAAGTCAACTTAACTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTTAAGTTGACTTTGAACTCGAGTTCAAAGTCAACTTAACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTTAAGTTGACTTTGAACTCGAGTTCAAAGTCAACTTAACTCGG-3'

Other clones with same target sequence:

(none)