Construct: shRNA TRCN0000029897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008978.1-803s1c1
DNA Barcode:
CGAGATATTCTTCCATATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpn20 (19256)
Gene Description:
protein tyrosine phosphatase, non-receptor type 20
Transcript:
RefSeq NM_008978.1 (NON-CURRENT)
Match location:
Position 803 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATATTCTTCCATATGAT
Hairpin Sequence:
5'-CCGG-CGAGATATTCTTCCATATGAT-CTCGAG-ATCATATGGAAGAATATCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATATTCTTCCATATGATCTCGAGATCATATGGAAGAATATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATATTCTTCCATATGATCTCGAGATCATATGGAAGAATATCTCG-3'

Other clones with same target sequence:

(none)