Construct: shRNA TRCN0000029923

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008980.1-2471s1c1
DNA Barcode:
CAGGAGTACATTGACGCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpra (19262)
Gene Description:
protein tyrosine phosphatase, receptor type, A
Transcript:
RefSeq NM_008980.1 (NON-CURRENT)
Match location:
Position 2471 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000029923)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGTACATTGACGCCTTT
Hairpin Sequence:
5'-CCGG-CAGGAGTACATTGACGCCTTT-CTCGAG-AAAGGCGTCAATGTACTCCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGTACATTGACGCCTTTCTCGAGAAAGGCGTCAATGTACTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGTACATTGACGCCTTTCTCGAGAAAGGCGTCAATGTACTCCTG-3'

Other clones with same target sequence:

(none)