Construct: shRNA TRCN0000030047

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019819.2-359s1c1
DNA Barcode:
TCAGATCACCTCCTCTCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp14 (56405)
Gene Description:
dual specificity phosphatase 14
Transcript:
RefSeq NM_019819.2 (NON-CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030047)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGATCACCTCCTCTCTCTT
Hairpin Sequence:
5'-CCGG-TCAGATCACCTCCTCTCTCTT-CTCGAG-AAGAGAGAGGAGGTGATCTGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGATCACCTCCTCTCTCTTCTCGAGAAGAGAGAGGAGGTGATCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGATCACCTCCTCTCTCTTCTCGAGAAGAGAGAGGAGGTGATCTGA-3'

Other clones with same target sequence:

(none)