Construct: shRNA TRCN0000030061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021345.1-1139s1c1
DNA Barcode:
CCTTCAAGTTTATCTCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hacd3 (57874)
Gene Description:
3-hydroxyacyl-CoA dehydratase 3
Transcript:
RefSeq NM_021345.1 (NON-CURRENT)
Match location:
Position 1139 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCAAGTTTATCTCGTAAT
Hairpin Sequence:
5'-CCGG-CCTTCAAGTTTATCTCGTAAT-CTCGAG-ATTACGAGATAAACTTGAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCAAGTTTATCTCGTAATCTCGAGATTACGAGATAAACTTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCAAGTTTATCTCGTAATCTCGAGATTACGAGATAAACTTGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000320262