Construct: shRNA TRCN0000030087
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_023587.1-669s1c1
- DNA Barcode:
- CCATCAGCTTACCTAACAAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hacd2 (70757)
- Gene Description:
- 3-hydroxyacyl-CoA dehydratase 2
- Transcript:
- RefSeq NM_023587.1 (NON-CURRENT)
- Match location:
- Position 669 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000030087)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCATCAGCTTACCTAACAAAT
- Hairpin Sequence:
-
5'-CCGG-CCATCAGCTTACCTAACAAAT-CTCGAG-ATTTGTTAGGTAAGCTGATGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATCAGCTTACCTAACAAATCTCGAGATTTGTTAGGTAAGCTGATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATCAGCTTACCTAACAAATCTCGAGATTTGTTAGGTAAGCTGATGG-3'
Other clones with same target sequence:
(none)
