Construct: shRNA TRCN0000030101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145441.2-846s1c1
DNA Barcode:
GCTCTTCCTTTCCTCAGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubxn2a (217379)
Gene Description:
UBX domain protein 2A
Transcript:
RefSeq NM_145441.2 (NON-CURRENT)
Match location:
Position 846 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTCCTTTCCTCAGGTTT
Hairpin Sequence:
5'-CCGG-GCTCTTCCTTTCCTCAGGTTT-CTCGAG-AAACCTGAGGAAAGGAAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTCCTTTCCTCAGGTTTCTCGAGAAACCTGAGGAAAGGAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTCCTTTCCTCAGGTTTCTCGAGAAACCTGAGGAAAGGAAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000314318