Construct: shRNA TRCN0000030342

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146898.1-382s1c1
DNA Barcode:
CCCTTGTACTACTCTTCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr1213 (258900)
Gene Description:
olfactory receptor 1213
Transcript:
RefSeq NM_146898.1 (NON-CURRENT)
Match location:
Position 382 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030342)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGTACTACTCTTCCATT
Hairpin Sequence:
5'-CCGG-CCCTTGTACTACTCTTCCATT-CTCGAG-AATGGAAGAGTAGTACAAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGTACTACTCTTCCATTCTCGAGAATGGAAGAGTAGTACAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGTACTACTCTTCCATTCTCGAGAATGGAAGAGTAGTACAAGGG-3'

Other clones with same target sequence:

(none)