Construct: shRNA TRCN0000030464

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198326.1-550s1c1
DNA Barcode:
GTAGTGCTAAAGCTCTGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsfl1c (386649)
Gene Description:
NSFL1 (p97) cofactor (p47)
Transcript:
RefSeq NM_198326.1 (NON-CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030464)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTGCTAAAGCTCTGGAAA
Hairpin Sequence:
5'-CCGG-GTAGTGCTAAAGCTCTGGAAA-CTCGAG-TTTCCAGAGCTTTAGCACTAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTGCTAAAGCTCTGGAAACTCGAGTTTCCAGAGCTTTAGCACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTGCTAAAGCTCTGGAAACTCGAGTTTCCAGAGCTTTAGCACTAC-3'

Other clones with same target sequence:

  1. TRCN0000297216