Construct: shRNA TRCN0000030526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011183.1-352s1c1
DNA Barcode:
CCACTATCAAGTCTGTGCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psen2 (19165)
Gene Description:
presenilin 2
Transcript:
RefSeq NM_011183.1 (NON-CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTATCAAGTCTGTGCGTT
Hairpin Sequence:
5'-CCGG-CCACTATCAAGTCTGTGCGTT-CTCGAG-AACGCACAGACTTGATAGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTATCAAGTCTGTGCGTTCTCGAGAACGCACAGACTTGATAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTATCAAGTCTGTGCGTTCTCGAGAACGCACAGACTTGATAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000277246