Construct: shRNA TRCN0000030529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010376.2-374s1c1
DNA Barcode:
CCAGGAGTACATCAACCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H13 (14950)
Gene Description:
histocompatibility 13
Transcript:
RefSeq NM_010376.2 (NON-CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAGTACATCAACCTCTT
Hairpin Sequence:
5'-CCGG-CCAGGAGTACATCAACCTCTT-CTCGAG-AAGAGGTTGATGTACTCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAGTACATCAACCTCTTCTCGAGAAGAGGTTGATGTACTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAGTACATCAACCTCTTCTCGAGAAGAGGTTGATGTACTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000297555