Construct: shRNA TRCN0000030680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023709.2-567s1c1
DNA Barcode:
GCCAAGCTTAACGGGAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Capn9 (73647)
Gene Description:
calpain 9
Transcript:
RefSeq NM_023709.2 (NON-CURRENT)
Match location:
Position 567 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGCTTAACGGGAGTTAT
Hairpin Sequence:
5'-CCGG-GCCAAGCTTAACGGGAGTTAT-CTCGAG-ATAACTCCCGTTAAGCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGCTTAACGGGAGTTATCTCGAGATAACTCCCGTTAAGCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGCTTAACGGGAGTTATCTCGAGATAACTCCCGTTAAGCTTGGC-3'

Other clones with same target sequence:

(none)