Construct: shRNA TRCN0000030714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016723.1-703s1c1
DNA Barcode:
TCTTGACAGAAACACCAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uchl3 (50933)
Gene Description:
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Transcript:
RefSeq NM_016723.1 (NON-CURRENT)
Match location:
Position 703 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTGACAGAAACACCAAATA
Hairpin Sequence:
5'-CCGG-TCTTGACAGAAACACCAAATA-CTCGAG-TATTTGGTGTTTCTGTCAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTGACAGAAACACCAAATACTCGAGTATTTGGTGTTTCTGTCAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTGACAGAAACACCAAATACTCGAGTATTTGGTGTTTCTGTCAAGA-3'

Other clones with same target sequence:

(none)