Construct: shRNA TRCN0000030715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016723.1-122s1c1
DNA Barcode:
CCTGAACTTCTTAGCATGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uchl3 (50933)
Gene Description:
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Transcript:
RefSeq NM_016723.1 (NON-CURRENT)
Match location:
Position 122 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAACTTCTTAGCATGGTA
Hairpin Sequence:
5'-CCGG-CCTGAACTTCTTAGCATGGTA-CTCGAG-TACCATGCTAAGAAGTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAACTTCTTAGCATGGTACTCGAGTACCATGCTAAGAAGTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAACTTCTTAGCATGGTACTCGAGTACCATGCTAAGAAGTTCAGG-3'

Other clones with same target sequence:

(none)