Construct: shRNA TRCN0000030737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013700.1-2590s1c1
DNA Barcode:
CCTGGGCTACATCTACTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp5 (22225)
Gene Description:
ubiquitin specific peptidase 5 (isopeptidase T)
Transcript:
RefSeq NM_013700.1 (NON-CURRENT)
Match location:
Position 2590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGCTACATCTACTTCTA
Hairpin Sequence:
5'-CCGG-CCTGGGCTACATCTACTTCTA-CTCGAG-TAGAAGTAGATGTAGCCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGCTACATCTACTTCTACTCGAGTAGAAGTAGATGTAGCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGCTACATCTACTTCTACTCGAGTAGAAGTAGATGTAGCCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000288536