Construct: shRNA TRCN0000030743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011678.1-348s1c1
DNA Barcode:
GCCTGGAATAAATTGCTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp4 (22258)
Gene Description:
ubiquitin specific peptidase 4 (proto-oncogene)
Transcript:
RefSeq NM_011678.1 (NON-CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGAATAAATTGCTGAAT
Hairpin Sequence:
5'-CCGG-GCCTGGAATAAATTGCTGAAT-CTCGAG-ATTCAGCAATTTATTCCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGAATAAATTGCTGAATCTCGAGATTCAGCAATTTATTCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGAATAAATTGCTGAATCTCGAGATTCAGCAATTTATTCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000288458