Construct: shRNA TRCN0000030761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009481.1-6464s1c1
DNA Barcode:
GCAGAAGAAATCACTATGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp9x (22284)
Gene Description:
ubiquitin specific peptidase 9, X chromosome
Transcript:
RefSeq NM_009481.1 (NON-CURRENT)
Match location:
Position 6464 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGAAATCACTATGATT
Hairpin Sequence:
5'-CCGG-GCAGAAGAAATCACTATGATT-CTCGAG-AATCATAGTGATTTCTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGAAATCACTATGATTCTCGAGAATCATAGTGATTTCTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGAAATCACTATGATTCTCGAGAATCATAGTGATTTCTTCTGC-3'

Other clones with same target sequence:

(none)