Construct: shRNA TRCN0000030785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144937.2-752s1c1
DNA Barcode:
GTGTCTTTAGTAGAAGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp3 (235441)
Gene Description:
ubiquitin specific peptidase 3
Transcript:
RefSeq NM_144937.2 (NON-CURRENT)
Match location:
Position 752 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCTTTAGTAGAAGAGTTT
Hairpin Sequence:
5'-CCGG-GTGTCTTTAGTAGAAGAGTTT-CTCGAG-AAACTCTTCTACTAAAGACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCTTTAGTAGAAGAGTTTCTCGAGAAACTCTTCTACTAAAGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCTTTAGTAGAAGAGTTTCTCGAGAAACTCTTCTACTAAAGACAC-3'

Other clones with same target sequence:

(none)