Construct: shRNA TRCN0000030840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_110937.4-1639s1c1
DNA Barcode:
CCAGTAATCAAGAATAGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp32 (237898)
Gene Description:
ubiquitin specific peptidase 32
Transcript:
RefSeq XM_110937.4 (NON-CURRENT)
Match location:
Position 1639 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAATCAAGAATAGCAAA
Hairpin Sequence:
5'-CCGG-CCAGTAATCAAGAATAGCAAA-CTCGAG-TTTGCTATTCTTGATTACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAATCAAGAATAGCAAACTCGAGTTTGCTATTCTTGATTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAATCAAGAATAGCAAACTCGAGTTTGCTATTCTTGATTACTGG-3'

Other clones with same target sequence:

(none)