Construct: shRNA TRCN0000030887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_149655.4-1559s1c1
DNA Barcode:
GCAACCAATGGCTGTGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp30 (100756)
Gene Description:
ubiquitin specific peptidase 30
Transcript:
RefSeq XM_149655.4 (NON-CURRENT)
Match location:
Position 1559 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCAATGGCTGTGGATTT
Hairpin Sequence:
5'-CCGG-GCAACCAATGGCTGTGGATTT-CTCGAG-AAATCCACAGCCATTGGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCAATGGCTGTGGATTTCTCGAGAAATCCACAGCCATTGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCAATGGCTGTGGATTTCTCGAGAAATCCACAGCCATTGGTTGC-3'

Other clones with same target sequence:

(none)