Construct: shRNA TRCN0000030890

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152825.1-1099s1c1
DNA Barcode:
GCATCTTTATTGGCGAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp45 (77593)
Gene Description:
ubiquitin specific petidase 45
Transcript:
RefSeq NM_152825.1 (NON-CURRENT)
Match location:
Position 1099 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030890)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTTTATTGGCGAATTAA
Hairpin Sequence:
5'-CCGG-GCATCTTTATTGGCGAATTAA-CTCGAG-TTAATTCGCCAATAAAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTTTATTGGCGAATTAACTCGAGTTAATTCGCCAATAAAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTTTATTGGCGAATTAACTCGAGTTAATTCGCCAATAAAGATGC-3'

Other clones with same target sequence:

(none)